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Arraystar inc human m6a epitranscriptomic microarray
Human M6a Epitranscriptomic Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+m6a+epitranscriptomic+microarray/human+mrna+lncrna+epitranscriptomic+microarray/pm40374016-89-11-19
Average 90 stars, based on 1 article reviews
human m6a epitranscriptomic microarray - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Transfection:

Article Title: METTL3-mediated m6A modification of SIRT1 mRNA inhibits progression of endometriosis by cellular senescence enhancing
Article Snippet: .. Wild-type ESCs and ESCs transfected with the oe-METTL3 plasmid were analysed by Aksomics Company (Shanghai, China) using a human m6A epitranscriptomic microarray and mRNA microarray at Arraystar m6A single-base resolution [ ]. ..

Plasmid Preparation:

Article Title: METTL3-mediated m6A modification of SIRT1 mRNA inhibits progression of endometriosis by cellular senescence enhancing
Article Snippet: .. Wild-type ESCs and ESCs transfected with the oe-METTL3 plasmid were analysed by Aksomics Company (Shanghai, China) using a human m6A epitranscriptomic microarray and mRNA microarray at Arraystar m6A single-base resolution [ ]. ..

Microarray:

Article Title: METTL3-mediated m6A modification of SIRT1 mRNA inhibits progression of endometriosis by cellular senescence enhancing
Article Snippet: .. Wild-type ESCs and ESCs transfected with the oe-METTL3 plasmid were analysed by Aksomics Company (Shanghai, China) using a human m6A epitranscriptomic microarray and mRNA microarray at Arraystar m6A single-base resolution [ ]. ..

Article Title: N6-methyladenosine regulates metabolic remodeling in kidney aging through transcriptional regulator GLIS1
Article Snippet: .. Human m6A epitranscriptomic microarray and mRNA microarray analyses were performed based on Arraystar’s standard protocols. ..

Article Title: WTAP-mediated m 6 A modification of lncRNA DIAPH1-AS1 enhances its stability to facilitate nasopharyngeal carcinoma growth and metastasis.
Article Snippet: As the most predominant RNA epigenetic regulation in eukaryotic cells, N-methyladenosine (mA) plays a critical role in human tumorigenesis and cancer progression.. However, the biological function and molecular mechanism of mA regulation in nasopharyngeal carcinoma (NPC) remain elusive.. Here, we showed that Wilms’ tumor 1-associating protein (WTAP) expression was apparently upregulated in NPC, and increased WTAP was associated with poor prognosis.

Article Title: METTL3 promotes human amniotic epithelial stem cells differentiation into insulin-producing cells by regulation of MaFA expression.
Article Snippet: Objective: Generating mature β-cells from stem cells remains a significant challenge in diabetes cell therapy.. Human amniotic epithelial stem cells (hAESCs) have made their mark in regenerative medicine, and provide several advantages compared to other stem cells.. Methyltransferase-like 3 (METTL3), an essential RNA methyltransferase participating in N6-methyladenosine (m6A) mRNA methylation, plays a critical role in the normal development of β-cells, yet its deletion in β-cells leads to β-cell dysfunction and hyperglycemia.

Article Title: Mettl14 Attenuates Cardiac Ischemia/Reperfusion Injury by Regulating Wnt1/β-Catenin Signaling Pathway
Article Snippet: .. After merging, it was hybridized to Arraystar Human m6A Epitranscriptomic Microarray (8 × 60 K, Arraystar). .. Finally, an Agilent scanner G2505C was used to scan the array.

Article Title: METTL3 promotes colorectal carcinoma progression by regulating the m6A–CRB3–Hippo axis
Article Snippet: The modified RNAs were eluted from the immunoprecipitated magnetic beads as the “IP.” The unmodified RNAs were recovered from the supernatant as “Sup.” The IP and Sup RNAs were labeled with Cy5 and Cy3, respectively, using the Arraystar Super RNA Labeling Kit. .. The RNAs were combined and hybridized onto an Arraystar Human m6A Epitranscriptomic Microarray (8x60K, Arraystar). .. After the slides were washed, the arrays were scanned using an Agilent Scanner G2505C.

Article Title: WTAP/YTHDF1-mediated m 6 A modification amplifies IFN-γ-induced immunosuppressive properties of human MSCs.
Article Snippet: .. In the hybridization step, the cRNA was merged to the Arraystar Human m6A Epitranscriptomic Microarray (8 60 K; Arraystar, Rockville, USA). .. Finally, the array was scanned using an Agilent G2505C scanner.

Labeling:

Article Title: WTAP-mediated m 6 A modification of lncRNA DIAPH1-AS1 enhances its stability to facilitate nasopharyngeal carcinoma growth and metastasis.
Article Snippet: As the most predominant RNA epigenetic regulation in eukaryotic cells, N-methyladenosine (mA) plays a critical role in human tumorigenesis and cancer progression.. However, the biological function and molecular mechanism of mA regulation in nasopharyngeal carcinoma (NPC) remain elusive.. Here, we showed that Wilms’ tumor 1-associating protein (WTAP) expression was apparently upregulated in NPC, and increased WTAP was associated with poor prognosis.

Hybridization:

Article Title: METTL3 promotes human amniotic epithelial stem cells differentiation into insulin-producing cells by regulation of MaFA expression.
Article Snippet: Objective: Generating mature β-cells from stem cells remains a significant challenge in diabetes cell therapy.. Human amniotic epithelial stem cells (hAESCs) have made their mark in regenerative medicine, and provide several advantages compared to other stem cells.. Methyltransferase-like 3 (METTL3), an essential RNA methyltransferase participating in N6-methyladenosine (m6A) mRNA methylation, plays a critical role in the normal development of β-cells, yet its deletion in β-cells leads to β-cell dysfunction and hyperglycemia.

Article Title: WTAP/YTHDF1-mediated m 6 A modification amplifies IFN-γ-induced immunosuppressive properties of human MSCs.
Article Snippet: .. In the hybridization step, the cRNA was merged to the Arraystar Human m6A Epitranscriptomic Microarray (8 60 K; Arraystar, Rockville, USA). .. Finally, the array was scanned using an Agilent G2505C scanner.



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Image Search Results


Primer sequences for key  LncRNA  and GAPDH.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: Primer sequences for key LncRNA and GAPDH.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Sequencing

Network of LncRNA-miRNA-mRNA was constructed to demonstrate possible regulatory relationships among lncRNAs, miRNAs, and mRNAs (the top 10 most significant target genes). Red represents LncRNAs, blue represents miRNAs, and green represents mRNAs.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: Network of LncRNA-miRNA-mRNA was constructed to demonstrate possible regulatory relationships among lncRNAs, miRNAs, and mRNAs (the top 10 most significant target genes). Red represents LncRNAs, blue represents miRNAs, and green represents mRNAs.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Construct

The expression levels of the four key LncRNAs in the 12 pairs of blood were quantified using qRT-PCR. Comparing with the control group, the expression levels of LncRNA FAR2, LncRNA LINC02431, and LncRNA AL357060.1 were downregulated, while the expression level of LncRNA FOXD2-AS1 was upregulated. ∗∗∗∗ p < 0.0001.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: The expression levels of the four key LncRNAs in the 12 pairs of blood were quantified using qRT-PCR. Comparing with the control group, the expression levels of LncRNA FAR2, LncRNA LINC02431, and LncRNA AL357060.1 were downregulated, while the expression level of LncRNA FOXD2-AS1 was upregulated. ∗∗∗∗ p < 0.0001.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Expressing, Quantitative RT-PCR, Control

The methylation levels of the four key LncRNAs in the 12 pairs of blood were quantified using MeRip-qPCR. Comparing with control group, the methylation levels of LncRNA FAR2, LncRNA FOXD2-AS1, and LncRNA AL357060.1 were hypomethylated, while the methylation level of LncRNA LINC02431 was not significantly different. ∗∗∗∗ p < 0.0001. ns: nonsignificant.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: The methylation levels of the four key LncRNAs in the 12 pairs of blood were quantified using MeRip-qPCR. Comparing with control group, the methylation levels of LncRNA FAR2, LncRNA FOXD2-AS1, and LncRNA AL357060.1 were hypomethylated, while the methylation level of LncRNA LINC02431 was not significantly different. ∗∗∗∗ p < 0.0001. ns: nonsignificant.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Methylation, Control